Coding

Part:BBa_M36306

Designed by: Raman Nelakanti, Dennis Te, Darren Jindal   Group: Stanford BIOE44 - S11   (2011-12-08)

FseI restriction endonuclease

The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|'

Used to cut specific DNA sequences that FseI recognizes. DOES NOT contain start codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 499
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None